23 datasets found

Delivering foreign molecules and genetic material into cells is a crucial process in life sciences and biotechnology, resulting in great interest in effective cell transfection...

Oxidation of the epigenetic DNA mark 5-methylcytosine by Tet dioxygenases is an established route to diversify the epigenetic information, modulate gene expression and overall...

Chromatin has been shown to undergo diffusional motion, and during active gene transcription the RNA polymerase activity negatively affects chromatin motion. However, the...

Pericentric heterochromatin (PCH) forms spatio-temporarily distinct compartments and affects chromosome organization and stability. Albeit some of its components are known, an...

DNA replication is a fundamental process ensuring the complete and accurate maintenance of the genome each time cells divide. This is particularly relevant early in development...

DNA organization on chromatin has been largely studied for its consequences in gene expression, but its contribution to the cell mechanics is often neglected despite the nucleus...

Heterochromatin is essential for nuclear integrity, genome stability, and gene regulation. However, the mechanisms governing heterochromatin compartmentalization remain poorly...

Heterochromatin is the highly compacted form of chromatin with various condensation levels hallmarked by high DNA methylation. MeCP2 is mostly known as a DNA methylation reader...

CTCF is a nuclear protein initially discovered for its role in enhancer-promoter insulation. It has been shown to play a role in genome architecture and in fact its DNA binding...

The accuracy of replication is one of the most important mechanisms ensuring stability of the genome. The fork protection complex prevents premature replisome stalling and/or...

Ultraviolet A and B (UVA 320-400 nm and UVB 280-320 nm) induced cyclobutane-pyrimidine dimers (CPDs) are the most critical lesions caused by environmental sun exposure. Here we...

Rett syndrome is a human intellectual disability disorder that is associated with mutations in the X-linked MECP2 gene. The epigenetic reader MeCP2 binds to methylated cytosines...

DNA base modifications diversify the genome and are essential players in development. Yet, their influence on DNA physical properties and the ensuing effects on genome...

Chromatin dynamics play a crucial role in cellular differentiation, yet tools for studying global chromatin mobility in living cells remain limited. Here, we developed a novel...

The dynamics of DNA in the cell nucleus plays a role in cellular processes and fates but the interplay of DNA mobility with the hierarchical levels of DNA organization is...

To ensure error-free duplication of all (epi)genetic information once per cell cycle, DNA replication follows a cell type and developmental stage specific spatio-temporal...

The flexibility of the spatio-temporal genome replication program during development and disease highlights the regulatory role of plastic epigenetic mechanisms over genetic...

MacroH2A has been linked to transcriptional silencing, cell identity, and is a hallmark of the inactive X chromosome (Xi). However, it remains unclear whether macroH2A plays a...

The dynamic regulation of RNA metabolism plays a crucial part in cellular function, with emerging evidence suggesting an important role for RNA modifications in this process....

All measurements derived from smFISH experiments as well as additional output from Bayesian inference are publicly via the institutional repository of Technical University...