87 datasets found

Sub-adult tissue pieces were processed into single cell RNA sequencing libraries in order to catalog spatial information and cell type distribution Overall design: Sub-adult...

The study goal was to identify cnidarian genes with circadian expression oscilation

Using ChIP-seq, we identified genes bound by pSMAD1/5 in Nematostella. RNA-Seq of BMP2/4 and GDF5-like morphants was used to verify the ChIP-Seq data.

Chromatin accessibility changes reveal key</p><p>regulatory aspects of the epigenomic landscape between 5 genes expression clusters</p><p>characterized.

Functional validation of Hyl1 like protein in Nematostella

The goal of this study is to enrich for stem cells in Hexacorallia by using ALDH activity as a marker. Nematostella vectensis cells were mechanically dissociated and stained...

Transcriptomes of Nematostella vectensis planulae NveAGO1 and NveAGO2 knockdowns.

We aimed to study the capacity of candidate stem cells in Nematostella vectensis to differentiate upon transplantation into a recipient animal. TBP::mCherry transgenic animals...

Classical embryological studies revealed that during mid-embryogenesis vertebrates show similar morphologies. This “phylotypic stage” has recently received support from...

We investigate the evolution of venom in Nematostella among populations by using a combination of transcriptomics, long-read genome sequencing, genomic qPCR and proteomics.

Short read Illumina paired end 76-100 bp transcript data from five developmental stages, adult male, and adult female.

Using forward transcriptomics, we show that beta-catenin/Tcf signaling and BMP2/4 signaling provide differential inputs into the cnidarian endomesodermal gene regulatory network...

Knockdown of NveAGO1 and NveAGO2 in Nematostella results in severe developmental defects. NveAGO1 and NveAGO2 immunoprecipitation reveals subfunctionalization for differential...

PacBio HIFI Reads for nematostella transcriptome assembly

To survive, organisms must deploy a range of molecular, physiological, and behavioral responses to avoid or counteract harmful environmental conditions. Often these responses to...

knockdown of HEN1 in Nematostella results in a developmental arrest. Small RNA sequencing revealed that the levels of both miRNAs and piRNAs drop dramatically in the morphant...

The identification of large numbers of single nucleotide polymorphisms (SNPs) throughout a genome has the power to refine the understanding of population demographic history and...

The goal of this study was to identify genome-wide-binding sites for the class IV POU homedomain transcription factor of the cnidarian Nematostella vectensis by using chromatin...

We exposed N. vectensis to three forms of trauma (streptomycin, cytochalasin D, or calcium-free seawater) to determine differences in gene expression during recovery from the...

No study to date has uncovered the complete transcriptomic landscape of cnidocytes or the venom producing cells of the phylum Cnidaria, impeding our understanding of the...