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Mutational_Signatures_in_the_YKO_collection
This project is being carried out through a Strategic Award from the Wellcome Trust to Steve Jackson, Mike Stratton, Peter Campbell, Serena Nik-Zainal and others. This grant is... -
Epistatic mutations in PUMA BH3 drive an alternate binding mode to potently a...
We used computational models to design focused combinatorial libraries to identify BH3 peptides that bind tightly and selectively to Bfl-1. The final library was predicted to be... -
Identification of non-coding transcripts regulated by Rap1 and other transcri...
Eukaryotic cells utilize several mechanisms to ensure that expression of aberrant non-coding RNAs is limited. Gene looping, chromatin modification or remodeling, and RNA... -
Hcr1/eIF3j is a 60S ribosomal subunit recycling accessory factor in vivo
Hcr1/eIF3j is a sub-stoichiometric subunit of eukaryotic initiation factor 3 (eIF3) that can dissociate the post-termination 40S ribosomal subunit from mRNA in vitro. We... -
Saccharomyces Cerevisiae sequence reads from hybrid laboratory evolution expe...
Mixed population samples and clonal isolates from 18 hybrid populations of S.Cerevisiae evolved at different rates of outcrossing, -
Saccharomyces cerevisiae BAL3215 Gene Expression Profiling - S.c_20 transcrip...
Saccharomyces cerevisiae BAL3215 Gene Expression Profiling. Cells grown in YPD medium -
Evolutionary divergence and selective utilization of transcription-coupled hi...
Various histone modifications, including acetylation and methylation, are widely associated with gene transcription, but their functional importance at individual genes, and the... -
Saccharomyces cerevisiae yHRWh92 hybrid Resequencing
Genome sequencing of Saccharomyces hybrid strain -
Free glycans derived from O-mannosylated glycoproteins suggest the presence o...
In eukaryotic cells, unconjugated oligosaccharides structurally related to N-glycans (FNGs) are generated either from misfolded N-glycoproteins destined for endoplasmic... -
Natural variation in non-coding regions underlying phenotypic diversity in bu...
Determining the different sources of heritable variation underlying quantitative traits in nature is currently at the forefront of genetic studies. To this end, molecular... -
Redesigning chromosomes for optimized Hi-C assay provides insights on loop fo...
Molecular proof of homologous pairing during meiosis prophase in S. cerevisiae, using a synthetic chromosomal region -
Identification of NAD+ capped mRNAs in S. cerevisiae
We report the identification of NAD-RNA in the yeast Saccharomyces cerevisiae under two distinct growth mediums. Overall design: RNA-seq libraries (in triplicate) were generated... -
DNCR1 single-site saturation mutagenesis
These data vary the interface residues of a designed chemically induced dimerizer. They show the residues allowed in the designed helical repeat protein scaffold, as well as... -
40S ribosome profiling reveals distinct roles for Tma20/Tma22 (MCT-1/DENR) an...
The recycling of ribosomes at stop codons for use in further rounds of translation is critical for efficient protein synthesis. Removal of the 60S subunit is catalyzed by the... -
DNA sequences of mutants with elevated yeast MAPK pathway reporter activity
Mitogen-activated protein kinase (MAPK) pathways control the response to intrinsic and extrinsic stimuli. In the budding yeast Saccharomyces cerevisiae , cells undergo... -
Saccharomyces cerevisiae 3' poly(A) site mapping
The use of alternative polyadenylation sites is common and affects the post-transcriptional fate of mRNA, including its stability, localization, and translation. Here we present... -
Assaying 6xIAPP toxicity
Measuring the change in gene expression profiles in yeast in response to 6xIAPP expression in high toxicity conditions Overall design: Cells were grown in 5 mL total volume in... -
Detection of splice isoforms and rare intermediates using multiplexed primer ...
Targeted RNA-sequencing aims to focus coverage on areas of interest that are inadequately sampled in standard RNA-sequencing experiments. Here we present a novel approach for... -
Saccharomyces cerevisiae Y06240 experimental evolution in YPD with 10% bottle...
Experimental evolution in batch, with different population bottlenecks, to determine the contribution of duplicated genes in biological innovation. Yeast line a1 was evolved in... -
Regulation of the Hsf1-dependent transcriptome via conserved bipartite contac...
Protein homeostasis and cellular fitness in the presence of proteotoxic stress is promoted by heat shock factor 1 (HSF1), which controls basal and stress-induced expression of...