Experiment on the influence of temperature, N:P supply ratio and pCO2 on elemental composition and growth rates of Emiliania huxleyi, supplement to: Bi, Rong; Ismar, Stefanie M; Sommer, Ulrich; Zhao, Meixun (2018): Simultaneous shifts in elemental stoichiometry and fatty acids of Emiliania huxleyi in response to environmental changes. Biogeosciences, 15(4), 1029-1045

DOI

Climate-driven changes in environmental conditions have significant and complex effects on marine ecosystems. Variability in phytoplankton elements and biochemicals can be important for global ocean biogeochemistry and ecological functions, while there is currently limited understanding on how elements and biochemicals respond to the changing environments in key coccolithophore species such as Emiliania huxleyi. We investigated responses of elemental stoichiometry and fatty acids (FAs) in a strain of E. huxleyi under three temperatures (12, 18 and 24 oC), three N:P supply ratios (molar ratios 10:1, 24:1 and 63:1) and two pCO2 levels (560 and 2400 µatm). Overall, C:N:P stoichiometry showed the most pronounced response to N:P supply ratios, with high ratios of particulate organic carbon vs. particulate organic nitrogen (POC:PON) and low ratios of PON vs. particulate organic phosphorus (PON:POP) in low N-media, and high POC:POP and PON:POP in low P-media. The ratio of particulate inorganic carbon vs. POC (PIC:POC) and polyunsaturated fatty acid proportions strongly responded to temperature and pCO2, both being lower under high pCO2 and higher with warming. We observed synergistic interactions between warming and nutrient deficiency (and high pCO2) on elemental cellular contents and docosahexaenoic acid (DHA) proportion in most cases, indicating the enhanced effect of warming under nutrient deficiency (and high pCO2). Our results suggest differential sensitivity of elements and FAs to the changes in temperature, nutrient availability and pCO2 in E. huxleyi, which is to some extent unique compared to non-calcifying algal classes. Thus, simultaneous changes of elements and FAs should be considered when predicting future roles of E. huxleyi in the biotic-mediated connection between biogeochemical cycles, ecological functions and climate change.

Identifier
DOI https://doi.org/10.1594/PANGAEA.887513
Related Identifier https://doi.org/10.5194/bg-15-1029-2018
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.887513
Provenance
Creator Bi, Rong; Ismar, Stefanie M; Sommer, Ulrich; Zhao, Meixun
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Publication Year 2018
Rights Creative Commons Attribution 3.0 Unported; https://creativecommons.org/licenses/by/3.0/
OpenAccess true
Representation
Language English
Resource Type Supplementary Dataset; Dataset
Format text/tab-separated-values
Size 1025 data points
Discipline Earth System Research