Seawater carbonate chemistry and sea urchin Paracentrotus lividus biological processes during experiments, 2011, supplement to: Martin, Sophie; Richier, Sophie; Pedrotti, Maria-Luiza; Dupont, Sam; Castejon, Charlotte; Gerakis, Yannis; Kerros, Marie-Emmanuelle; Oberhänsli, F; Teyssié, Jean-Louis; Jeffree, Ross; Gattuso, Jean-Pierre (2011): Early development and molecular plasticity in the Mediterranean sea urchin Paracentrotus lividus exposed to CO2-driven acidification. Journal of Experimental Biology, 214(8), 1357-1368

DOI

Ocean acidification is predicted to have significant effects on benthic calcifying invertebrates, in particular on their early developmental stages. Echinoderm larvae could be particularly vulnerable to decreased pH, with major consequences for adult populations. The objective of this study was to understand how ocean acidification would affect the initial life stages of the sea urchin Paracentrotus lividus, a common species that is widely distributed in the Mediterranean Sea and the NE Atlantic. The effects of decreased pH (elevated PCO2) were investigated through physiological and molecular analyses on both embryonic and larval stages. Eggs and larvae were reared in Mediterranean seawater at six pH levels, i.e. pHT 8.1, 7.9, 7.7, 7.5, 7.25 and 7.0. Fertilization success, survival, growth and calcification rates were monitored over a 3 day period. The expression of genes coding for key proteins involved in development and biomineralization was also monitored. Paracentrotus lividus appears to be extremely resistant to low pH, with no effect on fertilization success or larval survival. Larval growth was slowed when exposed to low pH but with no direct impact on relative larval morphology or calcification down to pHT 7.25. Consequently, at a given time, larvae exposed to low pH were present at a normal but delayed larval stage. More surprisingly, candidate genes involved in development and biomineralization were upregulated by factors of up to 26 at low pH. Our results revealed plasticity at the gene expression level that allows a normal, but delayed, development under low pH conditions.

Identifier
DOI http://dx.doi.org/doi:10.1594/PANGAEA.767600
Metadata Access http://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite3&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.767600
Provenance
Creator Pedrotti, Maria-Luiza;Gerakis, Yannis;Teyssié, Jean-Louis;Martin, Sophie;Gattuso, Jean-Pierre;Oberhänsli, F;Richier, Sophie;Kerros, Marie-Emmanuelle;Dupont, Sam;Castejon, Charlotte;Jeffree, Ross
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Publication Year 2011
Rights Creative Commons Attribution 3.0 Unported (CC-BY)
Representation
Language English
Resource Type Supplementary Dataset
Format text/tab-separated-values
Coverage
Discipline Earth System Research
Temporal Point 2009-04-14T11:59:59Z