This data set provides measurements of life history and morphological anti-predator responses of eight Daphnia galeata clones to the kairomones of two invertebrate predators, Leptodora kindtii and Bythotrephes longimanus. The first file “Octorina_etal_2022_life_history_responses.csv” provides data for age at first reproduction [days] and number of neonates per Daphnia [#/Daphnia] in 92 experimental units distributed across eight clones (A-H) and three treatments (Bythotrephes, Leptodora, and Control). The second file “Octorina_etal_2022_morphological_responses.csv” provides measurements of Daphnia body size [µm], body width [µm], head size [µm], spina size [µm], and eye diameter [µm] of 337 individuals measured in the 92 experimental units across the eight clones and three treatments. For more details see Octorina et al. (2022) Morphological defences and defence–cost trade‐offs in Daphnia in response to two co‐occurring invertebrate predators. Freshwater Biology, doi: https://doi.org/10.1111/fwb.13888