Oxygen respiration and metabolic enzyme malate dehydrogenase of Euphasia superba in the South Atlantic Ocean, supplement to: Pape, Carsten; Teschke, Mathias; Meyer, Bettina (2008): Melatonin and its possible role in mediating seasonal metabolic changes of Antarctic krill, Euphausia superba. Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, 149(4), 426-434

DOI

Melatonin, the chief secretory product of the vertebrate pineal gland is suspected to be a ubiquitous molecule principally involved in the transduction of photoperiodic information. Besides vertebrates, melatonin has been detected throughout phylogeny in numerous non-vertebrate taxa. In the present study, the occurrence of melatonin in Antarctic krill Euphausia superba and its possible role in mediating seasonal metabolic changes was evaluated. Melatonin was quantified by enzyme linked immunosorbent assay (ELISA) in high performance liquid chromatography (HPLC) purified extracts of eyestalks and hemolymph of krill sampled in the Lazarev Sea during the Antarctic winter and summer. In addition, oxygen uptake rates and the activities of the metabolic enzyme malate dehydrogenase (MDH) were recorded to assess the metabolic status of krill. Validation of melatonin measurements was carried out on the basis of three different extraction methods with parallel determination of melatonin by ELISA in crude extracts and in HPLC purified extracts, and after derivatization of melatonin under alkaline conditions in the presence of hydrogen peroxide. A significantly higher respiration rate and MDH activity was found in summer krill than in winter krill indicating that krill was in a state of reduced metabolic activity during winter. However, neither during winter nor during summer there were detectable melatonin concentrations in the visual system or hemolymph of krill. Based on these results, we question a mediating role of melatonin in the control of seasonal metabolic changes in Antarctic krill in particular and its physiological significance in krill in general. Project: Lazarev Sea Krill Study (LAKRIS), Funded by German Ministry of Education and Research (BMBF)

Identifier
DOI https://doi.org/10.1594/PANGAEA.667467
Related Identifier https://doi.org/10.1016/j.cbpa.2008.02.001
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.667467
Provenance
Creator Pape, Carsten; Teschke, Mathias; Meyer, Bettina
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Publication Year 2007
Rights Creative Commons Attribution 3.0 Unported
OpenAccess true
Representation
Language English
Resource Type Supplementary Collection of Datasets
Format application/zip
Size 14 datasets
Discipline Earth System Research
Spatial Coverage (-3.027W, -69.501S, 3.369E, -56.015N)
Temporal Coverage Begin 2005-12-07T03:44:00Z
Temporal Coverage End 2006-08-14T18:30:00Z