The dataset comprises data on the substrate type, temperature, salinity and benthos from 58 samples colected in Danube front area in the period March 1960 - July 1960, (Sulina-Portita sector, Romanian Black Sea Coast). The sediment samples were taken with a Van-Veen grab, the sampling size is was - 0.1 m2. The samples have been preserved with 10% buffered formalin, then sieved through a 2 mm, 0.25 mm and 0.10 mm mesh sieve and subsequently stored in 70% ethanol. The database comprises taxonomic and quantitative data (individuals count, density and wet weight biomass per square meter) for 95 macrobenthic species and 10 supraspecific groups.