Data provided is related to Task 3.4, Deliverable n°: 3.4 aimed at investigating the pretreatment of straw and other fibrous biomass prior to AD in order to improve the performance of conventional anaerobic digestion of recalcitrant fractions of agricultural waste, such as wheat straw. Moreover, bioethanol production from the anaerobically digested straw through the enzymatic utilization of the remaining fibre was also investigated in order to add value to the AD process through the exploitation of digestate as a bio-energy source.