140 discrete samples of dissolved organic carbon (DOC), Nitrate (NO3-), and Ammonium (NH4-) were collected exclusively at one site in the headwaters of the stream. Nutrient samples (including duplicates) were collected with a sample-rinsed 60 mL polyethylene syringe. Discrete samples for dissolved organic carbon (DOC) analysis were filtered using pre-combusted 0.7 µm GF/F filters (Whatman), into 40 mL borosilicate vials (USP Type I). Samples for nitrogen were immediately filtered using Satourious ™ 0.45μm cellulose acetate syringe filters into 10 mL polyethylene sample tubes. All samples were kept in a cold icebox, away from light, for less than 5 hours and then frozen until laboratory analysis. Nutrient analysis of DOC was performed with the 40 mL borosilicate vials (USP Type I) first treated with 30 µL of H3PO4 before analysis using an Aurora 1030W TOC Analyser (Thermo Fisher Scientific, ConFLo IV). Nutrient analysis of ammonium (NH4+) and nitrate (NO3-) was performed on the 10 mL polyethylene sample tubes colourimetrically using a Lachat Flow Injection Analyser.