The data involves annotations with the MBARI VARS annotation software of pelagic HD video transects obtained by the pelagic in situ observations system PELAGIOS. PELAGIOS is a newly developed towed camera system for deep-sea biological exploration and performance of video transects for diversity and distribution data. The data was collected in 2015 during cruise MSM49 on R/V MARIA S. MERIAN, from 20 to 950 m, during day (187 minutes) and night (292 minutes) transects on the northwestern slope of Senghor Seamount (17°14.2'N, 22°00.7'W; bottom depth of approximately 1000 m). The annotated organisms include fishes, crustaceans and gelatinous zooplankton. One file includes the transect length at each depth at day or night and another file has all individual annotated taxa observed at a particular depth at day or night. The Figure 4 is made with this data. A third file involves the data we used to make Figure 3 which is the comparison between the observations of Poeobius observed in PELAGIOS and UVP5 to calculate sample volume.