The long-term ecological research benthic monitoring comprises four representative permanent stations (SSd, Slt, FSd and WB) that have been sampled countinuously since 1969. The four stations are representative for the different benthic communities in the German Bight. Inter-annual variability and possible long-term trends were analysed based on spring-time samples since 1969. Earlier datasets have been published in the publication series https://doi.org/10.1594/PANGAEA.667646. Macrozoobenthos of soft-bottom benthic community was collected by van-Veen grabs. This dataset contains the continuation of this time series with the samples collected in spring between 2016 and 2019 each year in the North Sea, German Bight. Data for each campaign comprise four stations in the German Bight, sampled by grab samples (infauna). Biodiversity data of species include abundance (count data) and biomass (wet mass, g) per sample.