The dataset presents oxygen isotopic composition of benthic foraminifera Cibicides sp., planktonic foraminifera Globigerinoides ruber (white), and Globorotalia truncatulinoides (dextral), and Mg/Ca of G. truncatulinoides (dextral) over the last 300 ka in the western tropical Atlantic. The samples came from sediment core GL-1180 (8°S). Additionally, oxygen isotopic data of Globorotalia inflata from sediment core GL-1090 (southwestern Atlantic, 24°S) covering MIS 6 is also reported. This dataset was used to reconstruct the upper ocean stratification of the western tropical and subtropical South Atlantic over the late Pleistocene.