(Table 2) Age determination of harp seals (Pagophilus groenlandicus) from the Barents Sea - comparison of methods, supplement to: Garde, Eva; Frie, Anne K; Dunshea, Glenn; Hansen, Steen H; Kovacs, Kit Maureen; Lydersen, Christian (2010): Harp seal ageing techniques-teeth, aspartic acid racemization, and telomere sequence analysis. Journal of Mammalogy, 91(6), 1365-1374

DOI

Lower jaws (containing the teeth), eyes, and skin samples were collected from harp seals (Pagophilus groenlandicus) in the southeastern Barents Sea for the purpose of comparing age estimates obtained by 3 different methods, the traditional technique of counting growth layer groups (GLGs) in teeth and 2 novel approaches, aspartic acid racemization (AAR) in eye lens nuclei and telomere sequence analyses as a proxy for telomere length. A significant correlation between age estimates obtained using GLGs and AAR was found, whereas no correlation was found between GLGs and telomere length. An AAR rate (k Asp) of 0.00130/year ± 0.00005 SE and a D-enantiomer to L-enantiomer ratio at birth (D/L 0 value) of 0.01933 ± 0.00048 SE were estimated by regression of D/L ratios against GLG ages from 25 animals (12 selected teeth that had high readability and 13 known-aged animals). AAR could prove to be useful, particularly for ageing older animals in species such as harp seals where difficulties in counting GLGs tend to increase with age. Age estimation by telomere length did not show any correlation with GLG ages and is not recommended for harp seals.

Data extracted in the frame of a joint ICSTI/PANGAEA IPY effort, see http://doi.pangaea.de/10.1594/PANGAEA.150150

Identifier
DOI https://doi.org/10.1594/PANGAEA.810152
Related Identifier https://doi.org/10.1644/10-MAMM-A-080.1
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.810152
Provenance
Creator Garde, Eva; Frie, Anne K; Dunshea, Glenn; Hansen, Steen H; Kovacs, Kit Maureen; Lydersen, Christian
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Publication Year 2010
Rights Creative Commons Attribution 3.0 Unported
OpenAccess true
Representation
Language English
Resource Type Supplementary Dataset
Format text/tab-separated-values
Size 527 data points
Discipline Earth System Research
Spatial Coverage (40.167W, 68.400S, 45.583E, 70.200N); Barents Sea
Temporal Coverage Begin 2006-04-09T00:00:00Z
Temporal Coverage End 2006-04-24T00:00:00Z