During the cruise POS432 on board the German R/V Poseidon, we collected water with a CTD SBE 11plus equipped with 14 Hydrobios free flow bottles of 10 L each in 15 stations (resulting in almost 100 samples) in the Madeira basin region, Northeast Atlantic. We aimed to study the physical and biogeochemical conditions of the water column along the 22°W meridian, north and south of the Azores Front, in May 2012. We measured concentrations of chlorophyll a, phaeopigments, suspended particulate material (SPM), and nutrients such as nitrate, nitrite, phosphate, and silicate.