The prominent band induced by growth on L-cysteate and pyruvate was manually excised. After in-gel digestion with trypsin and extraction, the mixture of peptides was loaded onto Orbitrap Fusion LUMOS MS. The MS/MS spectra from each LC-MS/MS run were searched against the B. wadsworthia protein database GCF_000185705.2 (Bilo_- wads_3_1_6_V2) from UniProt (release date of Mar, 2022; 68,406 entries) using an in-house Proteome Discoverer (Version PD 2.2, Thermo-Fisher Scientific). Protein identifications were performed based on Sequest HT.

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