VFA functionalization through the addition of hydroxylic group by a bioelectrochemical approach


VFA functionalization by the addition of hydroxylic group in the acidic chain would allow consecutive production of alternative biopolyesters else than microbial PHAs. In particular, the addition of the -OH function in the terminal C (omega-position) was explored by testing the activity on octanoic acid of two alkane/fatty acids degrading bacteria in a bioelectrochemical system. Two different bacterial strains were used for bioconversion tests: Pseudomonas oleovorans ATCC® 29347 and Bacillus megaterium ATCC® 14581. Dedicated bioelectrochemical reactors (BES) were designed and set up: they consisted of two gastight borosilicate glass bottles (total volume of 420 mL each) separated by a 3 cm2 cross-sectional area through a Nafion® 117 proton exchange membrane (PEM). Two different types of monooxygenases capable to hydroxylate alkanes and fatty acids were used. While in open circuit conditions (i.e., controls with no potential applied) both strains produce only traces of difunctional intermediates of octanoic acid degradation, a significant increase of the octanoic acid bioconversion into difunctional intermediates could be obtained with the application of a cathodic potential of -0.4 V vs. SHE, in particular with P. oleovorans and under resting cells conditions (macronutrients limitation). However, no exploitable results were obtained so far, since the application of bioelectrochemical systems to provide reducing power for the hydroxylation of VFA chain was not effective enough and no significant hydroxylated acid production was observed.

DOI https://doi.org/10.15454/H5C9ZS
Metadata Access https://entrepot.recherche.data.gouv.fr/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=doi:10.15454/H5C9ZS
Creator Giulio Zanaroli; Agnese Lai; Andrea Nuzzo
Publisher Recherche Data Gouv
Contributor Giulio Zanaroli
Publication Year 2020
Funding Reference EU Horizon 2020 Research and Innovation programme 688338
Rights info:eu-repo/semantics/openAccess
OpenAccess true
Contact Giulio Zanaroli (University of Bologna)
Resource Type Dataset
Format application/vnd.openxmlformats-officedocument.wordprocessingml.document
Size 13862
Version 1.0
Discipline Life Sciences; Medicine