Lytic phages can infect and hijack host bacterium’s biochemical machinery to make copy after copy of itself until the bacterium bursts. A lot of Vibrio phages have been isolated and characterized, however until now little is known about how Vibrio cells response to phage invasion on global transcriptome level. Transcriptome profiling of V. alginolyticus during a lytic phage infection was first investigated through strand-specific RNA-Seq analysis. This work will contribute to our comprehensive understanding on molecule mechanism of phage infection and the interaction between phages and hosts.