Analysing the effect of cellular energy levels on codon-specific ribosome occupancy in vivo

The aim of this study is to evaluate how cellular energy levels shape codon-specific decoding and affect codon-mediated mRNA degradation. To this end we made use of HT5P-seq (Zhang & Pelechano, 2021, PMID:35474692) in S. cerevisiae to footprint the ribosome of co-translationally degraded mRNAs in vivo upon swift changes in the concentration of intracellular ATP and other energy metabolites. Overall design: HT5P-seq was performed in 5 different time points (3 replicates each time points), spanning 5 min before and 10 min after the addition of antimycin A to respiring yeast as described in Walther T et al. (PMID: 20087341)

Identifier
Source https://data.blue-cloud.org/search-details?step=~012FF91D4B9370D913042989415F4B4AB4EA0995027
Metadata Access https://data.blue-cloud.org/api/collections/FF91D4B9370D913042989415F4B4AB4EA0995027
Provenance
Instrument NextSeq 2000; ILLUMINA
Publisher Blue-Cloud Data Discovery & Access service; ELIXIR-ENA
Publication Year 2024
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science