This dataset provides information on soil chemistry and soil bulk density as part of the Grassworks project, which investigates the restoration of species-rich grasslands in Germany. Grasslands are globally threatened ecosystems, and the project aims to identify factors that contribute to successful restoration, focusing on ecological complexity and stakeholder engagement. Data was collected from 187 grassland sites across three regions in Northern, Central, and Southern Germany, each with distinct socio-economic and ecological characteristics. Sampling occurred between 2022 and 2023 and included 40–41 restored grassland sites and 20–25 reference sites (10–12 positive, 10–13 negative) per region. At each site in March or early April at each vegetation plot per subtransect, we took soil samples (pooled from six soil cores, 20 mm diameter) that were further pooled into one sample per site (24 in total) and analyzed for total soil organic carbon (SOC), total nitrogen content, pH, and soil texture. Additionally, soil bulk density was measured at vegetation plots per site, to enable future assessment of carbon sequestration over time. Soil and bulk density samples were taken at two depths: 0–10 and 10–30 cm.