3,781 datasets found

In eukaryotes, biogenesis of ribosomes requires folding and assembly of the precursor rRNA (pre-rRNA) with a large number of proteins and snoRNPs into huge RNA-protein...

Genome wide screens identified negative genetic interactions between several cofactors of the exosome nuclease complex and the Bre5-Ubp3 ubiquitin protease complex. RNA-binding...

We report an optimised low-input FAIRE-seq (Formaldehyde-Assisted Isolation of Regulatory Elements) procedure to assay chromatin accessibility from yeast colonies. Sensitivity,...

Evolution of anaerobic fungi involved acquisition of oxygen-independent pathways for synthesis of pyridine nucleotides and Coenzyme-A</p><p>Strain IMX2300 (fms1delta...

Identification of novel causative genes determining the complex trait of high ethanol tolerance in yeast using pooled-segregant whole-genome sequence analysis.

We characterize the translational and transcriptional programs induced by MetR and investigate the potential mechanisms through which methionine regulates gene expression, using...

Evolution for Growth Advantage in Stationary Phase (GASP) in eukaryotes as observed in Saccharomyces cerevisiae

Information that regulates gene expression is encoded throughout each gene but if different regulatory regions can be understood in isolation, or if they interact, is unknown....

Genome assembly and variant analysis of two Saccharomyces cerevisiae strains isolated from stingless bee pollen

Purpose: Pre-ribosomal RNA is cleaved at defined sites to release the mature ribosomal RNAs, but the functions of many ribosome biogenesis factors involved in 18S rRNA release...

RNAseq profiling of engineered S. cerevisiae during conversion of cellulosic sugars into biofuels Sample from Anaerobic bioreactor containing cellulosic media

We show that ligation-competent Okazaki fragments in Saccharomyces cerevisiae are sized according to the chromatin repeat. Using deep sequencing, we demonstrate that ligation...

We have performed mRNA sequencing (RNA-Seq) and ribosome profiling (Ribo-Seq) of baker's yeast grown in normal and oxidative stress conditions.

We describe construction of the 660 kilobase synthetic yeast chromosome XI (synXI) and reveal how synthetic redesign of non-coding DNA elements impact the cell. To aid...

Saccharomyces cerevisiae Raw sequence reads

RNAseq profiling of engineered S. cerevisiae during conversion of cellulosic sugars into biofuels Sample from Anaerobic bioreactor containing cellulosic media

Stress granules are mRNA-protein assemblies formed on nontranslating mRNAs. Stress granules are important in the stress response, related to neuronal mRNP granules, and aberrant...

Saccharomyces cerevisiae engineered with Cas-CLOVER and piggyBac to produce a yeast strain that produces GGT.566 RNAi that directly causes female lethality.

We obtained RNA polymerase II occupancy profiles across the genome of S.cerevisiae strains: Abf1 anchor-away cells after addition of rapamycin for different time points...

The Gypsy-like element Ty3 inserts proximal to the transcription start sites of genes transcribed by RNA polymerase 3 (RNAP3). In this study, a random-barcode Ty3 was used to...