3,781 datasets found

the genome-wide transcriptional responses to changed temperatures were comprehensive analyzed by transcriptome sequencing

In the yeast genome, a large proportion of nucleosomes occupy well-defined positions. While the contribution of chromatin remodelers and DNA binding proteins to maintain this...

Resequencing experiments of S. cerevisiae populations that have undergone transcriptional tuning

For a typical RNA polymerase (pol) II transcribed budding yeast gene, the 5' -end is characterized by a nucleosome-free region (NFR) immediate upstream of the transcription...

Genome wide screens identified negative genetic interactions between several cofactors of the exosome nuclease complex and the Bre5-Ubp3 ubiquitin protease complex. RNA-binding...

This project was designed to isolate Saccharomyces cerevisiae strains in a screen for improved tolerance to pretreated lignocellulosic biomass inhibitors. These inhibitors are...

pGAP1, pMEP2, pDAL80 :: GFP construct in HO locus for FY4, GAT1-1, GAT1-2, GAT1-3

We have combined biochemical purification of Mediator from chromatin with ChIP-sequencing to reveal Mediator occupupancy to DNA globally and to identify proteins interacting...

We develop a chemical cleavage method that releases single nucleosome dyad-containing fragments, allowing us to precisely map both single nucleosomes and linkers with high...

We report the genomic location of H3K4me2 and H3K4me3 in Saccharomyces cerevisiae by ChIP-Seq Overall design: Examination of H3K4me2 and H3K4me3 across the Saccharomyces...

Transcriptional response to UV radiation

Comparison of 3 different nucleosome ponsitions in response to 3 nitrogen conditions Overall design: Comparison of 3 different nucleosome ponsitions in response to 3 nitrogen...

wt and sua7-1 nascent mRNA profiling was performed using the GRO-seq approach. Br-UTP labelling of nascent RNA followed by immunoprecipitation and cDNA library preparation with...

RNA-seq data of Saccharomyces cerevisiae strain, NCIM3186 stress-treated transcriptomes

RNAseq profiling of engineered S. cerevisiae during conversion of cellulosic sugars into biofuels Sample from Anaerobic bioreactor containing cellulosic media

Saccharomyces cerevisiae rad27? strain was subcultured for 25 generations and sequenced using Illumina paired-end sequencing.

Libraries were prepared by using two methods. The one is ThruPlex kit from Rubicon Genomics, the other is TCS-ligation based method described in our paper. Overall design: Two...

RNAseq profiling of engineered S. cerevisiae during conversion of cellulosic sugars into biofuels Sample from Anaerobic bioreactor containing cellulosic media

RNAseq profiling of engineered S. cerevisiae during conversion of cellulosic sugars into biofuels Sample from Aerobic bioreactor containing cellulosic media

Cdc7 kinase is known to initiate DNA replication, but it is unknown where Cdc7 is found within the genome. We modified the Calling Cards method that uses the Ty5 retrotransposon...