Description of dataset

This dataset includes all necessary information to reproduce the figures from the publication "Microdevice for confinement of T-cells on functionalized bio-interface".

DOI: https://doi.org/10.1039/D5LC00248F

Article: Microdevice for confinement of T-cells on functionalized bio-interfaces

Authors

Christoph Trenzinger,a   Caroline Kopittke,a   Barbora Kalousková,a   Nemanja Šikanić,a   Marina Bishara,a   Gerhard J. Schütza  and  Mario Brameshubera

• Corresponding authorsa Institute of Applied Physics, TU Wien, Wiedner Hauptstraße 8-10, 1040 Vienna, Austria

Contact

E-mail: ctrenzinger@hotmail.com, brameshuber@iap.tuwien.ac.at

Technical details

Reproduction of the figures

Python: for all figures generated with Python, the corresponding Jupyter Notebook file is provided. The _readme.txt contains information about the files in the corresponding folder, requirements.txt contains information about the Python version and dependencies.

Matlab: the scripts to analyse data and to generate figures is provided in the corresponding folders. notes.txt files contain remarks for the analysis. All numerical data for analysis with Matlab is provided in CSV format.

Required Software

Matlab (Mathworks), tested with version 2019b

Cellprofiler (4.2.1), https://cellprofiler.org

Python (version 3.10), depencies are listed in the file requirements.txt

Licenses

Data is licensed under the Creative Commons Attribution 4.0 InternationalSoftware is licensed under the MIT license

Further notes

The .cpproj files are CellProfilerProject files, containing data in HDF5 format.The .czi files are Carl Zeiss Imaging formats, and can be read e.g.with the bio-formats plugin for ImageJ/Fiji.

Mechanical stimuli are an integral part of the natural cellular microenvironment, influencing cell growth, differentiation, and survival, particularly in mechanically challenging environments like tumors. These stimuli are also crucial in the T-cell microenvironment, where they play a role in antigen recognition and pathogen detection. To study T-cell mechanobiology effectively, in vitro methods must replicate these mechanical stimuli induced by compression, tension or shear flow, in the presence of antigen-presenting cells (APCs). While custom-made microdevices and microfluidic chips have successfully observed bulk cell behavior under mechanical strain, no existing device fully replicated the T-cell mechanoenvironment comprehensively. In this study, we developed a microdevice that integrates the mechanoenvironmental aspects of an APC mimicry with compression under live-cell imaging conditions. This device allows for precise confinement of cells between two glass surfaces, which can be individually coated with functional bio-interfaces. The microdevice is reusable and enables presetting of confinement heights, manual seeding of cells and the assembly of components directly at the microscope. To validate our microdevice we confined primary mouse T-cells on different APC-mimicking supported lipid bilayers while monitoring their morphology and migratory behaviour over time. To study the effect of confinement on TCR signalling, we tracked intracellular calcium levels and quantified Erk1/2 phosphorylation by immunostaining. We observed that T-cell morphology and motility are affected by confinement but also by bilayer composition. Moreover our findings suggest that confinement, despite not interfering with T-cell activation, might increase TCR background signalling in resting T-cells. Importantly, our microdevice is not limited to T-cell research; it can also serve as a platform for studying mechanical stimulation in other cell types, cell aggregates like spheroids and organoids, or even tissue samples in the presence of various bio-interfaces.