Comparison of samples and mock communities amplified with single-base variations in 515-Forward universal primer

This study is a comparison of marine environmental and marine mock community samples amplified with different versions of a commonly used universal forward primer 515. A mismatch in the 4th position in the 515F primer to the Thaumarchaea was corrected by replacing the (C) in that position with an ambiguity (N).

Identifier
Source https://data.blue-cloud.org/search-details?step=~0120F9066036350F2ED5C4DBB1127C60ABA51CDC259
Metadata Access https://data.blue-cloud.org/api/collections/0F9066036350F2ED5C4DBB1127C60ABA51CDC259
Provenance
Instrument 532; 308
Publisher Blue-Cloud Data Discovery & Access service; MGnify
Publication Year 2026
OpenAccess true
Contact blue-cloud-support(at)maris.nl
Representation
Discipline Marine Science
Spatial Coverage (-118.400W, 33.550S, -118.400E, 33.550N)
Temporal Point 2017-02-21T00:00:00Z