Gypsum Hill Spring is a cold saline spring flowing perennially in an area of thick permafrost in the Canadian high Arctic and an analogue for putative microbial habitats on extraterrestrial astrobiology targets such as Mars, Europa, and Enceladus. Metagenomic, metatranscriptomic, and amplicon sequencing were utilized to investigate and describe the active microbial community inhabiting the spring sediment.