Methane concentration and stable isotope ratios in seawater and sea ice, Utqiagvik shelf, Alaska


Arctic seawater was collected at two sites close to Utqiagvik, North Slope, Alaska on 7, 9, 11 and 15 April 2016 in the Beaufort Sea. Site "Elson Lagoon" (EL) was located North of Utqiagvik (7.4.2016, 71.334° N, -156.363° W), covered with 1.5 m thick sea ice and shallow with approximately 1.5 m depth. Water was collected from a depth of 1.5 m in a narrow layer of water between the sea ice and the sediment. Site "Ice Mass Balance Buoy" (IMB) was located 1 km offshore of Utqiagvik (7.4.2016, 71.373° N, -156.548° W, and 9.4.2016 - 15.4.2016, 71.372° N, -156.540° W). This site was characterized by 1 m thick fast ice cover and a water depth of approximately 7 m. Water was collected at five depths from 1 to 6.5 m.Sea ice was collected at station IMB on 11 and 15 April 2016 in the Beaufort Sea (71.372° N, -156.540° W) using a Kovacs Mark II ice corer (Kovacs, Roseburg, Oregon, USA). The ice cores were sectioned into 15 cm and split lengthwise. The core sections were sealed into custom-made gas-tight tubes for determination of methane concentration and isotope ratios. In the laboratory, the gas-tight tubes were flushed with ultrapure nitrogen for several gas volumes. Due to technical limitations, ice core 1 (IC1) was melted within a week at 5°C, while ice core 2 (IC2) was melted within a day, while frequently being mixed, at room temperature.Seawater temperature and salinity were recorded with an YSI Professional Plus probe (YSI, Ohio, USA) and a YSI 600 OMS V2 sonde (YSI, Ohio, USA). Water was collected using either a peristaltic pump (Masterflex Environmental Sampler, Cole Parmer, Illinois, USA) or submersible pump (Cyclone, Proactive Environmental Products, Florida, USA) from different water depths. Methane concentration and isotope ratios were determined with a Picarro G2201-i cavity ring-down spectrometer (Picarro, Santa Clara, California, USA) coupled to a Small Sample Isotope Module (SSIM) (Picarro, Santa Clara, California, USA), in a headspace approach. Nutrient concentrations were determined using a QuickChem QC8500 automated ion analyzer (Lachat, Loveland, Colorado, USA). The total number of prokaryotic cells was counted on a BD Influx^TM^ flow cytometer with BD FACS^TM^ software. Formol-fixed samples were stained with SYBR Green I (Invitrogen, Molecular Probes, Eugene, Oregon, USA) before analysis.

Supplement to: Uhlig, Christiane; Kirkpatrick, John B; d'Hondt, Steven L; Loose, Brice (2018): Methane-oxidizing seawater microbial communities from an Arctic shelf. Biogeosciences, 15, 3311-3329

Related Identifier
Metadata Access
Creator Uhlig, Christiane ; Kirkpatrick, John B; Loose, Brice
Publisher PANGAEA
Publication Year 2018
Rights Creative Commons Attribution 3.0 Unported;
OpenAccess true
Language English
Resource Type Supplementary Publication Series of Datasets; Collection
Format application/zip
Size 2 datasets
Discipline Earth System Research
Spatial Coverage (-156.548W, 71.334S, -156.363E, 71.373N)
Temporal Coverage Begin 2016-04-07T00:00:00Z
Temporal Coverage End 2016-04-15T00:00:00Z