Viral diversity and functional roles in subarctic permafrost thaw ponds (Whapmagoostui-Kuujjuarapik, Nunavik)

Viruses play an important role in aquatic ecosystems, where they cause the mortality and influence the evolution of their host cells. Viruses thereby are key players in nutrient regeneration in the ecosystems they reside. This research project aims to determine the viral diversity of thaw ponds in SAS, KWK and BGR sites. We will also determine whether viruses play a role in the methane cycle in those ponds, by either lysing methane-cycling cells or by the possession of auxilliary metabolic genes involved in this specific cycle. Surface and bottom water have been collected and filtered on 0.02µm pore-size filters (prefiltered on 0.2µm pore-size filters). DNA will be extracted and metagenomic librairies will be constructed to identify the viral genes. Network analysis will be performed to link viral taxonomic abundance with that of their hosts. Flow cytometry samples have also been taken and analysed for viral as well as cellular abundance. Ultimately any difference in viral diversity and functions among the different lakes sampled, will be assessed.

Identifier
Source https://www.polardata.ca/pdcsearch/PDCSearchDOI.jsp?doi_id=12776
Metadata Access http://www.polardata.ca/oai/provider?verb=GetRecord&metadataPrefix=fgdc&identifier=12776_fgdc
Provenance
Creator Culley, Alexander; Mohit, Vani; Deshpande, Bethany; Lévesque, Alice; Matveev, Alex; Vincent, Warwick
Publisher Canadian Cryospheric Information Network
Contributor Polar Data Catalogue
Publication Year 2017
Rights Research programs, CCIN, or ArcticNet take no liability for the use or transmission of this data
OpenAccess true
Contact warwick.vincent(at)bio.ulaval.ca; pdc(at)uwaterloo.ca
Representation
Language English
Format Computer file
Discipline Ecology
Spatial Coverage (-77.800W, 55.300S, -76.600E, 56.000N)
Temporal Coverage Begin 2014-08-15T00:00:00Z
Temporal Coverage End 9999-12-31T00:00:00Z