Supplemental Data underlying the publication: Uncovering the signaling landscape controlling breast cancer cell migration identifies novel metastasis driver genes

TNBC is an aggressive and highly metastatic breast cancer subtype. Enhanced TNBC cell motility is a prerequisite of TNBC cell dissemination. Here we apply an imaging-based RNAi phenotypic cell migration screen using two highly motile TNBC cancer cell lines (Hs578T and MDA-MB-231) to provide a repository of signaling determinants that functionally drive TNBC cell motility. We have screened ~4,200 target genes individually and discovered 133 and 113 migratory modulators of Hs578T and MDA-MB-231, respectively, which are linked to signaling networks predictive for breast cancer progression. The splicing factors PRPF4B and BUD31 and the transcription factor BPTF are essential for cancer cell migration, amplified in human primary breast tumors and associated with metastasis-free survival. Depletion of PRPF4B, BUD31 and BPTF causes primarily down-regulation of genes involved in focal adhesion and ECM-interaction pathways. PRPF4B is essential for TNBC metastasis formation in vivo, making PRPF4B a candidate for further drug development.

Identifier
DOI https://doi.org/10.17026/dans-z33-fgx2
PID https://nbn-resolving.org/urn:nbn:nl:ui:13-ar-p5vt
Metadata Access https://easy.dans.knaw.nl/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:easy.dans.knaw.nl:easy-dataset:129122
Provenance
Creator Koedoot, E
Publisher Data Archiving and Networked Services (DANS)
Publication Year 2019
Rights info:eu-repo/semantics/restrictedAccess; DANS License; https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf
OpenAccess false
Representation
Language English
Resource Type Dataset
Format application/pdf; .ods; .avi
Discipline Life Sciences