Dataset Figure_1: Determination of Whi5 concentration with widefield fluorescence microscopy may be confounded by photobleaching

DOI

This dataset contains all data and scripts used to generate figure 1, "Determination of Whi5 concentration with widefield fluorescence microscopy may be confounded by photobleaching".

  • The ImageJ macro ("Macro_bleaching.ijm") is used to background correct the unprocessed bleaching movies, which are stored in the folders "Bleaching GFP (movies)" and "Bleaching RFP (movies)" respectively.
  • The Python notebook ("Fig1_data_analysis.ipynb") contains all code needed to analyze the bleaching movies and generate figure 1.
Identifier
DOI https://doi.org/10.34894/SWRBYR
Metadata Access https://dataverse.nl/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=doi:10.34894/SWRBYR
Provenance
Creator Litsios, Athanasios ORCID logo; Goswami, Pooja; Terpstra, Hanna M.; Coffin, Carleton; Vuillemenot, Luc-Alban ORCID logo; Rovetta, Mattia; Ghazal, Ghada; Guerra, Paolo ORCID logo; Buczak, Katarzyna ORCID logo; Schmidt, Alexander; Tollis, Sylvain; Tyers, Mike ORCID logo; Royer, Catherine A. ORCID logo; Milias-Argeitis, Andreas ORCID logo; Heinemann, Matthias ORCID logo
Publisher DataverseNL
Contributor Groningen Digital Competence Centre; DataverseNL
Publication Year 2022
Rights CC0 Waiver; info:eu-repo/semantics/openAccess; https://creativecommons.org/publicdomain/zero/1.0/
OpenAccess true
Contact Groningen Digital Competence Centre (University of Groningen)
Representation
Resource Type Dataset
Format text/plain; application/x-7z-compressed
Size 1437; 543912748
Version 1.0
Discipline Life Sciences; Medicine