Seawater carbonate chemistry and sperm motility of Tegillarca granosavia

DOI

Since the industrial revolution, massive amount of anthropogenic carbon dioxide (CO2) have been generated to elevate the atmospheric CO2 concentration. Some anthropogenic CO2 have been absorbed by the ocean to cause “ocean acidification” (OA). Although the negative impacts of OA on sperm motility are increasingly found in various marine invertebrate species, the cellular and molecular mechanisms for these effects are still poorly understood. This study investigated the effect of OA (pH7.8 and 7.4) on sperm motility and energy supplying pathway in blood clam Tegillarca granosa. The results showed that the sperm swimming speed reduced significantly in acidified seawater. Since the adenosine triphosphate (ATP) level of sperm is closely related to its motility, we analyzed the sperm ATP content and activities of key enzymes during ATP synthesis under different OA scenarios. OA treatments significantly reduced ATP content as well as activities of 6-phosphofructokinase and pyruvate kinase in the sperm of T. granosa. The sperm Ca2+-ATPase of various animals has been reported to regulate sperm motility. Therefore, we explored the Ca2+-ATPase activity of T. granosa sperm under OA treatment. The results found that Ca2+-ATPase activities in the sperm of T. granosa were significantly declined under OA scenarios. In conclusion, these results suggested that OA could constrain sperm motility through inhibiting ATP synthesis and disturbing intracellular Ca2+ regulation.

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2021-03-15.

Identifier
DOI https://doi.org/10.1594/PANGAEA.929195
Related Identifier https://doi.org/10.7541/2020.070
Related Identifier https://cran.r-project.org/web/packages/seacarb/index.html
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.929195
Provenance
Creator Shi, Wei; Han, Yu; Huang, Xianke; Hu, Lihua; Chai, Xueliang; Liu, Guangxu
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Contributor Yang, Yan
Publication Year 2021
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Representation
Language English
Resource Type Dataset
Format text/tab-separated-values
Size 1263 data points
Discipline Earth System Research
Spatial Coverage (121.244 LON, 28.284 LAT)