The factors influencing prolactin (PRL) variation in birds and in wildlife in general have rarely been investigated with respect to the physiological impacts of exposure to environmental contaminants. We investigated the associations between circulating baseline PRL levels and concentrations of eight persistent organohalogen contaminant (OHC) classes (i.e., major organochlorines and brominated flame retardants, and associated metabolic products) in blood (plasma) of free-ranging glaucous gulls (Larus hyperboreus), a top predator in the Norwegian Arctic, engaged in the process of incubation. We further examined whether plasma OHC concentrations were associated with the variation of PRL in glaucous gulls exposed to a standardized capture/restraint protocol. Plasma OHC concentrations in male glaucous gulls were 2-to 3-fold higher relative to females. Baseline PRL levels tended to be higher in females compared to males, although not significantly (p = 0.20). In both males and females, the 30-min capture/restraint protocol led on average to a 26% decrease in PRL levels, which resulted in a rate of PRL decrease of 0.76 ng/mL/min. The baseline PRL levels and the rate of decrease in PRL levels tended to vary negatively with plasma OHC concentrations in males, but not in females, although several of these associations did not adhere with the criterion of significance (alpha = 0.05). Present results suggest that in highly OHC-exposed male glaucous gulls, the control of PRL release may be affected by the direct or indirect modulating actions of OHCs and/or their metabolically derived products. We conclude that potentially OHC-mediated impact on PRL secretion in glaucous gulls (males) may be a contributing factor to the adverse effects observed on the reproductive behavior, development and population size of glaucous gulls breeding in the Norwegian Arctic.
Concentrations per gram wet weight.Sum4CBz: sum of 1,2,4,5-TeCBz, 1,2,3,4-TeCBz, PnCBz and HxCBz.Sum6CHL: sum of heptachlor epoxide, oxychlordane, trans-chlordane, cis-chlordane, trans-nonachlor and cis-nonachlor. Sum3DDT: sum of p,p'-DDT, p,p'-DDE and p,p'-DDD.Sum58PCBs: sum of CB22, 28/31, 20/33,41/64,42,44,47/48,49, 52, 56/60, 66, 70/76, 74, 85, 87, 90/101, 92, 95, 97, 99,105,110,114,118,128,130,137,138,141,146,149,151,153, 156, 157, 158, 167, 170/190, 171, 172, 174, 176, 177, 178, 179, 180, 183, 187, 189, 194, 195,196/203, 199, 200, 202, 206, 207 and 208.Sum28OH-PCBs: sum of 4'-OH-CB79, 4-OH-CB97, 4-OH-CB107/4'-OH-CB108, 2'-OH-CB114, 4'-OH-CB120, 4'-OH-CB127, 4'-OH-CB130, 4-OH-CB134, 4-OH-CB146, 4'-OH-CB159, 4-OH-CB162, 4-OH-CB163, 4'-OH-CB177, 4-OH-CB178, 3'-OH-CB180, 3'-OH-CB182, 3'-OH-CB183, 3'-OH-CB184, 4-OH-CB187, 4-OH-CB193, 4'-OH-CB198, 4'-OH-CB199, 4'-OH-CB200, 4'-OH-CB201, 4'-OH-CB202, 4,4'-diOH-CB202, 3'-OH-CB203 and 4'-OH-CB208.Sum15MeSO2-PCBs: sum of 3-MeSO2-CB49, 4-MeSO2-CB49, 3-MeSO2-CB52, 4-MeSO2-CB52, 4-MeSO2-CB64, 3-MeSO2-CB70, 4-MeSO2-CB70, 4-MeSO2-CB87, 3-MeSO2-CB101, 4-MeSO2-CB101, 3-MeSO2-CB110, 4-MeSO2-CB110, 3-MeSO2-CB132, 4-MeSO2-CB132 and 3-MeSO2-CB149.Sum38PBDE: sum of BDE17, 25, 28, 47, 49, 54, 66, 75, 77, 85, 99, 100, 116, 119, 138, 139, 140, 153, 154/BB153, 155, 156, 171, 180, 181, 183, 184, 190, 191, 196, 197, 201, 202, 203, 205, 206, 207, 208 and 209.Sum15MeO-PBDE: sum of 6'-MeO-BDE17,4'-MeO-BDE17, 2'-MeO-BDE28, 4-MeO-BDE42, 5-MeO-BDE47, 6-MeO-BDE47, 3-MeO-BDE47,4'-MeO-BDE49, 6'-MeO-BDE49, 2'-MeO-BDE68, 6-MeO-BDE85, 6-MeO-BDE90, 6-MeO-BDE99, 2-MeO-BDE123 and 6-MeO-BDE137. Data extracted in the frame of a joint ICSTI/PANGAEA IPY effort, see http://doi.pangaea.de/10.1594/PANGAEA.150150
Supplement to: Verreault, Jonathan; Verboven, Nanette; Gabrielsen, Geir W; Letcher, Robert J; Chastel, Olivier (2008): Changes in prolactin in a highly organohalogen contaminated Arctic top predator seabird, the glaucous gull. General and Comparative Endocrinology, 156(3), 569-576
