Eukaryotic microbial community at the LTER site Helgoland Roads from March 2016 to March 2019

DOI

Water samples were collected from the long-term ecological research (LTER) site at Helgoland and the eukaryotic microbial community was assessed. 18S V4 region was amplified using the primer set 528iF /964iR and amplicon sequencing was performed on an Illumina MiSeq™ sequencer in a 2 × 300 bp paired-end run. Sequence data have been deposited in the European Nucleotide Archive (ENA) at the European Bioinformatics Institute (EMBL-EBI) under accession number PRJEB37135 using the data brokerage service of the German Federation for Biological Data (GFBio). 21 million sequences remained after bioinformatic processing and were clustered into Operational Taxonomic Units (OTUs). The Protist Ribosomal Reference database (PR2), version 4.11.1 was used as reference database.

Identifier
DOI https://doi.pangaea.de/10.1594/PANGAEA.921026
Related Identifier https://doi.org/10.1371/journal.pone.0244817
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.921026
Provenance
Creator Käse, Laura; Kraberg, Alexandra Claudia; Metfies, Katja; Neuhaus, Stefan; Sprong, Pim; Fuchs, Bernhard M; Boersma, Maarten; Wiltshire, Karen Helen
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Publication Year 2020
Rights Data access is restricted (moratorium, sensitive data, license constraints)
OpenAccess false
Representation
Language English
Resource Type Dataset
Format text/tab-separated-values
Size 2 data points
Discipline Earth System Research
Spatial Coverage (7.900 LON, 54.188 LAT); German Bight, North Sea