Heterotrophic nanoflagellate and bacteria biomass as carbon in water samples collected at Station MARCH-1997-GN36199704601MSB02 in the Aegean Sea

DOI

Subsamples (10-30 ml) were concentrated on 25 mm black polycarbonate filters (pore size 0.2 µm), stained with DAPI for 10 min (Porter, Feig, 1980) and filtered. Under epifluorescence microscopy heterotrophic bacteria were counted using UV excitation.Subsamples (10-30 ml) were concentrated on 25 mm black polycarbonate filters (pore size 0.6 µm), stained with DAPI for 10 min (Porter, Feig, 1980) and filtered. Under epifluorescence microscopy, heterotrophic (HF), photototrophic (PF) and mixotrophic (MF) nanoflagellates were distinguished using UV and blue excitation and enumerated. Nanoflagellates were classified in size categories and the biovolume was calculated.

Identifier
DOI https://doi.org/10.1594/PANGAEA.684680
Related Identifier https://doi.org/10.1594/PANGAEA.758079
Related Identifier https://doi.org/10.3354/meps181297
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.684680
Provenance
Creator Christaki, Urania; Van Wambeke, France; Dolan, John
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Contributor Hellenic Center of Marine Research, Institut of Oceanography, Greece
Publication Year 1999
Funding Reference Fourth Framework Programme; Sixth Framework Programme
Rights Creative Commons Attribution 3.0 Unported
OpenAccess true
Representation
Language English
Resource Type Dataset
Format text/tab-separated-values
Size 12 data points
Discipline Earth System Research
Spatial Coverage (25.100 LON, 35.745 LAT)