Temperature and acidification variability reduce physiological performance in the intertidal zone porcelain crab Petrolisthes cinctipes, supplement to: Paganini, Adam W; Miller, Nathan A; Stillman, Jonathon H (2014): Temperature and acidification variability reduce physiological performance in the intertidal zone porcelain crab Petrolisthes cinctipes. Journal of Experimental Biology, 217(22), 3974-3980

DOI

We show here that increased variability of temperature and pH synergistically negatively affects the energetics of intertidal zone crabs. Under future climate scenarios, coastal ecosystems are projected to have increased extremes of low tide-associated thermal stress and ocean acidification-associated low pH, the individual or interactive effects of which have yet to be determined. To characterize energetic consequences of exposure to increased variability of pH and temperature, we exposed porcelain crabs, Petrolisthes cinctipes, to conditions that simulated current and future intertidal zone thermal and pH environments. During the daily low tide, specimens were exposed to no, moderate or extreme heating, and during the daily high tide experienced no, moderate or extreme acidification. Respiration rate and cardiac thermal limits were assessed following 2.5 weeks of acclimation. Thermal variation had a larger overall effect than pH variation, though there was an interactive effect between the two environmental drivers. Under the most extreme temperature and pH combination, respiration rate decreased while heat tolerance increased, indicating a smaller overall aerobic energy budget (i.e. a reduced O2 consumption rate) of which a larger portion is devoted to basal maintenance (i.e. greater thermal tolerance indicating induction of the cellular stress response). These results suggest the potential for negative long-term ecological consequences for intertidal ectotherms exposed to increased extremes in pH and temperature due to reduced energy for behavior and reproduction.

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne et al, 2014) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation is 2014-12-09.

Identifier
DOI https://doi.org/10.1594/PANGAEA.840590
Related Identifier https://doi.org/10.1242/jeb.109801
Related Identifier https://cran.r-project.org/package=seacarb
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.840590
Provenance
Creator Paganini, Adam W; Miller, Nathan A; Stillman, Jonathon H
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Contributor Yang, Yan
Publication Year 2014
Rights Creative Commons Attribution 3.0 Unported; https://creativecommons.org/licenses/by/3.0/
OpenAccess true
Representation
Language English
Resource Type Supplementary Dataset; Dataset
Format text/tab-separated-values
Size 6924 data points
Discipline Earth System Research
Spatial Coverage (-123.244 LON, 38.514 LAT)
Temporal Coverage Begin 2012-09-01T00:00:00Z
Temporal Coverage End 2013-01-31T00:00:00Z