Seawater carbonate chemistry and the bacterial community present in larvae and spat of Crassostrea gigas

DOI

Changes in marine environments, including pH changes, have been correlated to alterations in the physiology and disease susceptibility of cultured organisms at the early stages of development. In this study, high-throughput sequencing of the V3-V4 region of the 16S rRNA gene was performed to evaluate the bacterialbiodiversity of Crassostrea gigas pediveliger larvae and spat under acidic stress compared to that of larvae at normal pH value. The evaluation was performed in an experimental system with continuous water flow and pHmanipulation by CO2 bubbling to simulate acidification (pH 7.38 ± 0.039), using the current ocean pH conditions (pH 8.116 ± 0.023) as a reference. The results indicated that the bacterial communities associated with both pediveliger larvae and spat were modified in response to acidic conditions. The families Rhodobacteraceae and Campylobacteraceae were the most affected by the change in pH, with increases in Vibrionaceae in pediveliger larvae and Planctomycetaceae and Phyllobacteriaceae in spat detected. The results of this study demonstrate that the bacterial communities associated with C. gigas pediveliger larvae and spat are responsive to changes in ocean acidification

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-03-06.

Identifier
DOI https://doi.org/10.1594/PANGAEA.913332
Related Identifier https://doi.org/10.3856/vol47-issue3-fulltext-13
Related Identifier https://CRAN.R-project.org/package=seacarb
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.913332
Provenance
Creator Flores-Higuera, Francisco A; Wördenweber, Robin; Luis-Villaseñor, Irasema E; Rochin-Arenas, Jesús A; Gómez-Gil, Bruno; Mazón-Suástegui, José Manuel; Voltolina, D
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Contributor Yang, Yan
Publication Year 2019
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Representation
Language English
Resource Type Dataset
Format text/tab-separated-values
Size 384 data points
Discipline Earth System Research