Microbial cell numbers measured during POLARSTERN cruise PS94 (TransArcII) to the Barents Sea and central Arctic

DOI

For the determination of prokaryotic cell numbers, 1 cm³ sediment was fixed with sterile filtered formalin/seawater at a final concentration of 2% and stored at 4°C until further analyses in the home laboratory. Samples were filtered on 0.2 µm polycarbonate filters, stained with acridine orange (Meyer-Reil, 1983, doi:10.1007/bf00395813), and counted using an epifluorescence microscope (Axiophot II Imaging, Zeiss, Jena, Germany). For each sample, 30 random grids from two replicate filters were counted and averaged.

Identifier
DOI https://doi.org/10.1594/PANGAEA.907884
Related Identifier https://doi.org/10.1594/PANGAEA.907889
Related Identifier https://doi.org/10.3389/fmars.2020.00426
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.907884
Provenance
Creator Bienhold, Christina ORCID logo
Publisher PANGAEA
Contributor Kiesel, Joshua
Publication Year 2019
Funding Reference Federal Ministry of Education and Research https://doi.org/10.13039/501100002347 Crossref Funder ID 03G0833E https://foerderportal.bund.de/foekat/jsp/SucheAction.do?actionMode=view&fkz=03G0833E Verbundprojekt: WTZ RUS: System Laptev-See: TRANSDRIFT; Seventh Framework Programme https://doi.org/10.13039/100011102 Crossref Funder ID 294757 https://cordis.europa.eu/project/id/294757 Assessment of bacterial life and matter cycling in deep-sea surface sediments
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Representation
Language English
Resource Type Dataset
Format text/tab-separated-values
Size 48 data points
Discipline Earth System Research
Spatial Coverage (-121.751W, 72.734S, 22.826E, 89.916N); Barents Sea; Arctic Ocean
Temporal Coverage Begin 2015-08-18T20:46:00Z
Temporal Coverage End 2015-10-08T10:20:00Z