Exp. II: cercarial activity and survival


For cercarial activity and survival infected periwinkles were acclimated for three weeks under the different treatments (n=10 individuals per treatment) and incubated to allow for cercarial release. At the start of the experiment, approximately 45 cercariae per treatment were added individually to wells of three 96-well plates The 96-well plates were then exposed to the different temperature treatments placing them in the six thermobaths (three 96-well plates per treatment combination). Activity and survival were then assessed under a stereomicroscope after 4, 6, 9, 18, 27, and 45 hours. Cercariae were considered fully active when they were constantly swirling around (category: fully active). When laying at the bottom and not reacting, these were considered dead (category: dead), when still reacting, even if slowly, these were still considered alive (category: alive). This last category included "fully active".

DOI https://doi.org/10.1594/PANGAEA.914100
Related Identifier https://doi.org/10.1594/PANGAEA.914102
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.914100
Creator Bommarito, Claudia; Pansch, Christian; Khosravi, Maral; Pranovi, Fabio; Wahl, Martin; Thieltges, David W
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Publication Year 2020
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Language English
Resource Type Dataset
Format text/tab-separated-values
Size 2600 data points
Discipline Earth System Research