Total RNA was purified from E. pacifica using with an RNeasy Lipid tissue mini kit. The library of E. pacifica for next generation sequencing was made using with a TruSeq RNA library prep kit v2 (Illumina). RNA purification and library preparation were performed according to the manufacturers’ instructions. The library was analyzed by Miseq using a Miseq reagent kit v3 (600 cycle) (Illumina). The fastaq data was assembled by Trinity.