Seawater carbonate chemistry and crab Necora puber size and elements in tissue during experiments, 2010, supplement to: Small, Daniel; Calosi, Piero; White, Daniel; Spicer, John I; Widdicombe, Stephen (2010): Impact of medium-term exposure to CO2 enriched seawater on the physiological functions of the velvet swimming crab Necora puber. Aquatic Biology, 10(1), 11-21

DOI

Ocean acidification (OA) is predicted to play a major role in shaping species biogeography and marine biodiversity over the next century. We tested the effect of medium-term exposure to OA (pH 8.00, 7.30 and 6.70 for 30 d) on acid-base balance in the decapod crab Necora puber-a species that is known to possess good extracellular buffering ability during short-term exposure to hypercapnic conditions. To determine if crabs undergo physiological trade-offs in order to buffer their haemolymph, we characterised a number of fundamental physiological functions, i.e. metabolic rate, tolerance to heat, carapace and chelae [Ca2+] and [Mg2+], haemolymph [Ca2+] and [Mg2+], and immune response in terms of lipid peroxidation. Necora puber was able to buffer changes to extracellular pH over 30 d exposure to hypercapnic water, with no evidence of net shell dissolution, thus demonstrating that HCO3- is actively taken up from the surrounding water. In addition, tolerance to heat, carapace mineralization, and aspects of immune response were not affected by hypercapnic conditions. In contrast, whole-animal O2uptake significantly decreased with hypercapnia, while significant increases in haemolymph [Ca2+] and [Mg2+] and chelae [Mg2+] were observed with hypercapnia. Our results confirm that most physiological functions in N. puber are resistant to low pH/hypercapnia over a longer period than previously investigated, although such resistance comes at the expenses of metabolic rates, haemolymph chemistry and chelae mineralization.

Identifier
DOI http://dx.doi.org/doi:10.1594/PANGAEA.778503
Metadata Access http://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite3&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.778503
Provenance
Creator Spicer, John I;Small, Daniel;Calosi, Piero;Widdicombe, Stephen;White, Daniel
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Publication Year 2010
Rights Creative Commons Attribution 3.0 Unported (CC-BY)
Representation
Language English
Resource Type Supplementary Dataset
Format text/tab-separated-values
Coverage
Discipline Earth System Research