Seawater carbonate chemistry and performance and oxidative status in a tolerant burrowing clam

DOI

Whereas low levels of thermal stress, irradiance, and dietary restriction can have beneficial effects for many taxa, stress acclimation remains understudied in marine invertebrates, despite being threatened by climate change stressors such as ocean acidification. To test for life-stage and stress-intensity dependence in eliciting enhanced tolerance under subsequent stress encounters, we initially conditioned pediveliger Pacific geoduck (Panopea generosa) larvae to (i) ambient and moderately elevated pCO2 (920 µatm and 2800 µatm, respectively) for 110 days, (ii) secondarily applied a 7-day exposure to ambient, moderate, and severely elevated pCO2 (750 µatm, 2800 µatm, and 4900 µatm, respectively), followed by 7 days in ambient conditions, and (iii) implemented a 7-day third exposure to ambient (970 µatm) and moderate pCO2 (3000 µatm). Initial conditioning to moderate pCO2 stress followed by second and third exposure to severe and moderate pCO2 stress increased respiration rate, organic biomass, and shell size suggesting a stress-intensity-dependent effect on energetics. Additionally, stress-acclimated clams had lower antioxidant capacity compared to clams under ambient conditions, supporting the hypothesis that stress over postlarval-to-juvenile development affects oxidative status later in life. Time series and stress intensity-specific approaches can reveal life-stages and magnitudes of exposure, respectively, that may elicit beneficial phenotypic variation.

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2021) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2022-2-24. 'EH' and 'AH' represents the ambient pCO2 history ('AH') or moderately-elevated pCO2 history ('EH') during the acclimation period prior to the repeat exposures. Characters after the 'history (AH or EH) represent pCO2 levels during the second exposure (A = ambient, M = moderately-elevated, and S = severely-elevated) and the third exposure (A = ambient, M = moderately-elevated). For instance, a row as 'EHSA' means moderately-elevated pCO2 acclimation, second exposure to severely-elevated pCO2, and third exposure to ambient pCO2. If an exposure is not given then that measurement was taken before the treatment was relevant.

Identifier
DOI https://doi.org/10.1594/PANGAEA.941416
Related Identifier https://doi.org/10.1242/jeb.233932
Related Identifier https://doi.org/10.5281/zenodo.3903019
Related Identifier https://cran.r-project.org/web/packages/seacarb/index.html
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.941416
Provenance
Creator Gurr, Samuel J; Trigg, Shelly A; Vadopalas, Brent; Pastore, P; Putnam, H M
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Contributor Yang, Yan
Publication Year 2021
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Representation
Language English
Resource Type Dataset
Format text/tab-separated-values
Size 39178 data points
Discipline Earth System Research
Temporal Coverage Begin 2019-07-04T00:00:00Z
Temporal Coverage End 2019-08-14T00:00:00Z