Seawater carbonate chemistry and morphometric, protein, and lipid data of Strongylocentrotus purpuratus, supplement to: Wong, Juliet M; Kozal, Logan C; Leach, Terence S; Hoshijima, Umihiko; Hofmann, Gretchen E (2019): Transgenerational effects in an ecological context: Conditioning of adult sea urchins to upwelling conditions alters maternal provisioning and progeny phenotype. Journal of Experimental Marine Biology and Ecology, 517, 65-77

DOI

Transgenerational plasticity occurs when the conditions experienced by the parental generation influence the phenotype of their progeny. This may in turn affect progeny performance and physiological tolerance, providing a means by which organisms cope with rapid environmental change. We conditioned adult purple sea urchins, Strongylocentrotus purpuratus, to combined pCO2 and temperature conditions reflective of in situ conditions of their natural habitat, the benthos in kelp forests of nearshore California, and then assessed the performance of their progeny raised under different pCO2 levels. Adults were conditioned during gametogenesis to treatments that reflected static non-upwelling (~650 μatm pCO2, ~17 °C) and upwelling (~1300 μatm pCO2, ~13 °C) conditions. Following approximately 4 months of conditioning, the adults were spawned and embryos were raised under low pCO2 (~450 μatm pCO2) or high pCO2 (~1050 μatm pCO2) treatments to determine if differential maternal conditioning impacted the progeny response to a single abiotic stressor: pCO2. We examined the size, protein content, and lipid content of eggs from both sets of conditioned female urchins. Offspring were sampled at four stages of early development: hatched blastula, gastrula, prism, and echinopluteus. This resulted in four sets of offspring: (1) progeny from non-upwelling-conditioned mothers raised under low pCO2, (2) progeny from non-upwelling-conditioned mothers raised under high pCO2, (3) progeny from upwelling-conditioned mothers raised under low pCO2, and (4) progeny from upwelling-conditioned mothers raised under high pCO2. We then assessed the effects of maternal conditioning along with the effects of developmental pCO2 levels on body size of the progeny. Our results showed that differential maternal conditioning had no impact on average egg size, although non-upwelling females produced eggs that were more variable in size. Maternal conditioning did not affect protein content but did have a modest impact on egg lipid content. Developing embryos whose mothers were conditioned to simulated upwelling conditions (~1300 μatm pCO2, ~13 °C) were greater in body size, although this effect was no longer evident at the echinopluteus larval stage. Although maternal conditioning affected offspring body size, the pCO2 levels under which the embryos were raised did not. Overall, this laboratory study provides insight into how transgenerational effects may function in nature. The impacts of parental environmental history on progeny phenotype during early development have important implications regarding recruitment success and population-level effects.

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-02-03.

Identifier
DOI https://doi.org/10.1594/PANGAEA.911802
Related Identifier https://doi.org/10.1016/j.jembe.2019.04.006
Related Identifier https://doi.org/10.5061/dryad.4nv0nb8
Related Identifier https://CRAN.R-project.org/package=seacarb
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.911802
Provenance
Creator Wong, Juliet M; Kozal, Logan C; Leach, Terence S; Hoshijima, Umihiko; Hofmann, Gretchen E
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Contributor Yang, Yan
Publication Year 2019
Rights Creative Commons Attribution 4.0 International; https://creativecommons.org/licenses/by/4.0/
OpenAccess true
Representation
Language English
Resource Type Supplementary Dataset
Format text/tab-separated-values
Size 114403 data points
Discipline Earth System Research
Spatial Coverage (-119.829 LON, 34.414 LAT)
Temporal Coverage Begin 2011-02-01T00:00:00Z
Temporal Coverage End 2011-02-28T00:00:00Z