Supplemental Data underlying the manuscript: Unraveling the spliceosomal control of breast cancer cell motility behavior

There is increasing evidence for a role of alternative splicing in the progression and metastasis of breast cancer. The role of the splicing machinery in the tumor cell migration of aggressive triple-negative breast cancer (TNBC) remains elusive. Here we systematically evaluated the role of RNA splicing in TNBC cell motility behavior. A targeted RNAi screen of all 244 major splicing factors in two highly motile TNBC cancer cell lines uncovered ten splicing factors, including CWC15, PHF5A, SF3A3, SMNDC1, SNRPA1, SNRPB2, SNRPD2, SNRPG, SRSF7 and U2AF1, that act as common migratory modulators in TNBC with limited effects on cell proliferation and survival. All of these factors were associated with aggressive breast cancer subtypes and/or metastasis-free survival in breast cancer patients. Two of the most prominent factors, SRSF7 and SMNDC1, are critical for RNA processing in association with regulating the expression of gene networks involved in cell-matrix adhesion signaling. SRSF7 resided in a large protein-protein interaction splicing complex together with PRPF4B and BUD31, and was affecting similar exon inclusion events, making this complex a candidate for drug development.

Identifier
DOI https://doi.org/10.17026/dans-xs9-hf76
PID https://nbn-resolving.org/urn:nbn:nl:ui:13-1n-bb7d
Metadata Access https://easy.dans.knaw.nl/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=oai:easy.dans.knaw.nl:easy-dataset:129211
Provenance
Creator Koedoot, E
Publisher Data Archiving and Networked Services (DANS)
Publication Year 2019
Rights info:eu-repo/semantics/restrictedAccess; DANS License; https://dans.knaw.nl/en/about/organisation-and-policy/legal-information/DANSLicence.pdf
OpenAccess false
Representation
Language English
Resource Type Dataset
Format application/pdf
Discipline Life Sciences