Dataset Figure_4: Nuclear Whi5-GFP intensity versus time from repeated imaging of the same individual cells

Dataset

DOI

Whi5-GFP intensity as a function of time for the same FOVs. Only small daughter cells in the asynchronous population at time point 0 were quantified as a function of time.

The folder 'Image Files' contains raw image files for Whi5-GFP for FOV1,3,4,5 & 6 at each of the 6 time points (0, 20, 40, 60, 80, 100 min).

The folder 'Excel_analysis_output' contains the output files for all five FOV (1,3,4,5,6) for 1. fovX_t0 or time 100_whi5_nadh_.xlsx – at time 0 and time 100 minutes. These files correspond to the analysis of the background auto-fluorescence excited at 750 nm used to calculate size. The only relevant information from these analyses is the Cyto size (fL) column in tab 3 of each file 2. fovX_tX_whi5_.xlsx Analysis of all time points and all FOV for Whi5-GFP intensities.

Identifier
DOI	https://doi.org/10.34894/IVWACK
Metadata Access	https://dataverse.nl/oai?verb=GetRecord&metadataPrefix=oai_datacite&identifier=doi:10.34894/IVWACK

Provenance
Creator	Litsios, Athanasios ; Goswami, Pooja; Terpstra, Hanna M.; Coffin, Carleton; Vuillemenot, Luc-Alban ; Rovetta, Mattia; Ghazal, Ghada; Guerra, Paolo ; Buczak, Katarzyna ; Schmidt, Alexander; Tollis, Sylvain; Tyers, Mike ; Royer, Catherine A. ; Milias-Argeitis, Andreas ; Heinemann, Matthias
Publisher	DataverseNL
Contributor	Groningen Digital Competence Centre; DataverseNL
Publication Year	2022
Rights	CC0 Waiver; info:eu-repo/semantics/openAccess; https://creativecommons.org/publicdomain/zero/1.0/
OpenAccess	true
Contact	Groningen Digital Competence Centre (University of Groningen)

Representation
Resource Type	Dataset
Format	text/plain; application/x-7z-compressed
Size	801; 3967826
Version	1.0
Discipline	Life Sciences; Medicine
Spatial Coverage	Troy NY 12180, USA