Seawater carbonate chemistry and processes during experiments with Emiliania huxleyi (TW1), 2003, supplement to: Sciandra, Antoine; Harlay, Jérôme; Lefèvre, Dominique; Lemee, R; Rimmelin, Peguy; Denis, Michel; Gattuso, Jean-Pierre (2003): Response of coccolithophorid Emiliania huxleyi to elevated partial pressure of CO2 under nitrogen limitation. Marine Ecology Progress Series, 261, 111-122

DOI

Precipitation of calcium carbonate by phytoplankton in the photic oceanic layer is an important process regulating the carbon cycling and the exchange of CO2 at the ocean-atmosphere interface. Previous experiments have demonstrated that, under nutrient-sufficient conditions, doubling the partial pressure of CO2 (pCO2) in seawater-a likely scenario for the end of the century-can significantly decrease both the rate of calcification by coccolithophorids and the ratio of inorganic to organic carbon production. The present work investigates the effects of high pCO2 on calcification by the coccolithophore Emiliania huxleyi (Strain TW1) grown under nitrogen-limiting conditions, a situation that can also prevail in the ocean. Nitrogen limitation was achieved in NO3-limited continuous cultures renewed at the rate of 0.5 d-1 and exposed to a saturating light level. pCO2 was increased from 400 to 700 ppm and controlled by bubbling CO2-rich or CO2-free air into the cultures. The pCO2 shift has a rapid effect on cell physiology that occurs within 2 cell divisions subsequent to the perturbation. Net calcification rate (C) decreased by 25% and, in contrast to previous studies with N-replete cultures, gross community production (GCP) and dark community respiration (DCR) also decreased. These results suggest that increasing pCO2 has no noticeable effect on the calcification/photosynthesis ratio (C/P) when cells of E. huxleyi are NO3-limited.

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI).

Identifier
DOI https://doi.org/10.1594/PANGAEA.727841
Related Identifier https://doi.org/10.3354/meps261111
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.727841
Provenance
Creator Sciandra, Antoine; Harlay, Jérôme; Lefèvre, Dominique; Lemee, R; Rimmelin, Peguy; Denis, Michel; Gattuso, Jean-Pierre
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Contributor Nisumaa, Anne-Marin
Publication Year 2003
Funding Reference Seventh Framework Programme, 211384; Sixth Framework Programme, 511106
Rights Creative Commons Attribution 3.0 Unported; https://creativecommons.org/licenses/by/3.0/
OpenAccess true
Representation
Language English
Resource Type Supplementary Dataset; Dataset
Format text/tab-separated-values
Size 941 data points
Discipline Earth System Research
Temporal Coverage Begin 2001-10-29T09:00:00Z
Temporal Coverage End 2001-11-24T08:00:00Z