Seawater carbonate chemistry and Strongylocentrotus purpuratus body length and gene expression pattern changes during experiments, 2011, supplement to: Stumpp, Meike; Dupont, Sam; Thorndyke, Mike; Melzner, Frank (2011): CO2 induced seawater acidification impacts sea urchin larval development II: Gene expression patterns in pluteus larvae. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 160(3), 320-330

DOI

Extensive use of fossil fuels is leading to increasing CO2 concentrations in the atmosphere and causes changes in the carbonate chemistry of the oceans which represents a major sink for anthropogenic CO2. As a result, the oceans' surface pH is expected to decrease by ca. 0.4 units by the year 2100, a major change with potentially negative consequences for some marine species. Because of their carbonate skeleton, sea urchins and their larval stages are regarded as likely to be one of the more sensitive taxa. In order to investigate sensitivity of pre-feeding (2 days post-fertilization) and feeding (4 and 7 days post-fertilization) pluteus larvae, we raised Strongylocentrotus purpuratus embryos in control (pH 8.1 and pCO2 41 Pa e.g. 399 µatm) and CO2 acidified seawater with pH of 7.7 (pCO2 134 Pa e.g. 1318 µatm) and investigated growth, calcification and survival. At three time points (day 2, day 4 and day 7 post-fertilization), we measured the expression of 26 representative genes important for metabolism, calcification and ion regulation using RT-qPCR.

Identifier
DOI http://dx.doi.org/doi:10.1594/PANGAEA.774447
Metadata Access http://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite3&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.774447
Provenance
Creator Melzner, Frank;Dupont, Sam;Stumpp, Meike;Thorndyke, Mike
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Publication Year 2011
Rights Creative Commons Attribution 3.0 Unported (CC-BY)
Representation
Language English
Resource Type Supplementary Dataset
Format text/tab-separated-values
Coverage
Discipline Earth System Research