Seawater carbonate chemistry and hatching, survival and development rate of Calanus pacificus, supplement to: McLaskey, Anna K; McElhany, Paul; Busch, Shallin D; Maher, Michael; Winans, Amanda K; Keister, Julie E (2019): Early life stages of Calanus pacificus are neither exposed nor sensitive to low pH waters. Journal of Plankton Research, fbz059


We characterized the vertical distribution of Calanus pacificus eggs and larvae and the carbonate chemistry that they are exposed to in Puget Sound, WA. We found that, under stratified conditions, more than 90% of eggs and nauplii stages 1–4 were distributed above the pycnocline, in seawater with pH higher than 7.7. In addition, eggs and larvae from 101 females were reared for 5 days under a range of pH conditions (7.2–8.0) to investigate how pH sensitivity varies among individuals. We observed a slight increase in naupliar survival at pH 7.3 in Individual Brood experiments, while in Mixed Brood experiments, exposure to pH 7.3 led to a small decline in hatching success. In a Split Brood experiment, inter-individual variability among different females' broods masked pH effects. These results indicate that C. pacificus early life stages are generally tolerant to short-term direct effects of ocean acidification.

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2019-12-20.

Related Identifier
Related Identifier
Metadata Access
Creator McLaskey, Anna K; McElhany, Paul; Busch, Shallin D; Maher, Michael; Winans, Amanda K; Keister, Julie E
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Contributor Yang, Yan
Publication Year 2019
Rights Creative Commons Attribution 4.0 International;
OpenAccess true
Language English
Resource Type Supplementary Dataset; Dataset
Format text/tab-separated-values
Size 368 data points
Discipline Earth System Research
Spatial Coverage (-123.090 LON, 48.600 LAT)
Temporal Coverage Begin 2013-08-01T00:00:00Z
Temporal Coverage End 2013-08-31T00:00:00Z