Seawater carbonate chemistry and larval growth response of the Portuguese oyster (Crassostrea angulata) in a laboratory experiment, supplement to: Thiyagarajan, Vengatesen; Ko, W K Ginger (2012): Larval growth response of the Portuguese oyster (Crassostrea angulata) to multiple climate change stressors. Aquaculture, 370-371, 90-95


Rising anthropogenic carbon dioxide (CO2) dissolving into coastal waters is decreasing the pH and carbonate ion concentration, thereby lowering the saturation state of calcium carbonate (CaCO3) minerals through a process named ocean acidification (OA). The unprecedented threats posed by such low pH on calcifying larvae of several edible oyster species have not yet been fully explored. Effects of low pH (7.9, 7.6, 7.4) on the early growth phase of Portuguese oyster (Crassostrea angulata) veliger larvae was examined at ambient salinity (34 ppt) and the low-salinity (27 ppt) treatment. Additionally, the combined effect of pH (8.1, 7.6), salinity (24 and 34 ppt) and temperature (24 °C and 30 °C) was examined using factorial experimental design. Surprisingly, the early growth phase from hatching to 5-day-old veliger stage showed high tolerance to pH 7.9 and pH 7.6 at both 34 ppt and 27 ppt. Larval shell area was significantly smaller at pH 7.4 only in low-salinity. In the 3-factor experiment, shell area was affected by salinity and the interaction between salinity and temperature but not by other combinations. Larvae produced the largest shell at the elevated temperature in low-salinity, regardless of pH. Thus the growth of the Portuguese oyster larvae appears to be robust to near-future pH level (> 7.6) when combined with projected elevated temperature and low-salinity in the coastal aquaculture zones of South China Sea.

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-03-24.

Related Identifier
Related Identifier
Metadata Access
Creator Thiyagarajan, Vengatesen; Ko, W K Ginger
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Contributor Yang, Yan
Publication Year 2012
Rights Creative Commons Attribution 3.0 Unported
OpenAccess true
Language English
Resource Type Supplementary Dataset
Format text/tab-separated-values
Size 405 data points
Discipline Earth System Research
Spatial Coverage (119.796 LON, 26.098 LAT)
Temporal Coverage Begin 2011-09-01T00:00:00Z
Temporal Coverage End 2011-11-30T00:00:00Z