Seawater carbon chemistry and calcification,carbonic anhydrase activity of cold-water coral Lophelia pertusa, supplement to: Kurman, Melissa; Gómez, C E; Georgian, Samuel E; Lunden, Jay J; Cordes, Erik E (2017): Intra-Specific Variation Reveals Potential for Adaptation to Ocean Acidification in a Cold-Water Coral from the Gulf of Mexico. Frontiers in Marine Science, 4

DOI

Ocean acidification, the decrease in seawater pH due to the absorption of atmospheric CO2, profoundly threatens the survival of a large number of marine species. Cold-water corals are considered to be among the most vulnerable organisms to ocean acidification because they are already exposed to relatively low pH and corresponding low calcium carbonate saturation states (Omega). Lophelia pertusa is a globally distributed cold-water scleractinian coral that provides critical three-dimensional habitat for many ecologically and economically significant species. In this study, four different genotypes of L. pertusa were exposed to three pH treatments (pH=7.60, 7.75, and 7.90) over a short (two-week) experimental period, and six genotypes were exposed to two pH treatments (pH=7.60, and 7.90) over a long (six-month) experimental period. Their physiological response was measured as net calcification rate and the activity of carbonic anhydrase, a key enzyme in the calcification pathway. In the short-term experiment, net calcification rates did not significantly change with pH, although they were highly variable in the low pH treatment, including some genotypes that maintained positive net calcification in undersaturated conditions. In the six-month experiment, average net calcification was significantly reduced at low pH, with corals exhibiting net dissolution of skeleton. However, one of the same genotypes that maintained positive net calcification (+0.04% day-1) under the low pH treatment in the short-term experiment also maintained positive net calcification longer than the other genotypes in the long-term experiment, although none of the corals maintained positive calcification for the entire 6 months. Average carbonic anhydrase activity was not affected by pH, although some genotypes exhibited small, insignificant, increases in activity after the sixth month. Our results suggest that while net calcification in L. pertusa is adversely affected by ocean acidification in the long term, it is possible that some genotypes may prove to be more resilient than others, particularly to short perturbations of the carbonate system. These results provide evidence that populations of L. pertusa in the Gulf of Mexico may contain the genetic variability necessary to support an adaptive response to future ocean acidification.

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2017-07-19.

Identifier
DOI https://doi.org/10.1594/PANGAEA.877984
Related Identifier https://doi.org/10.3389/fmars.2017.00111
Related Identifier https://cran.r-project.org/package=seacarb
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.877984
Provenance
Creator Kurman, Melissa; Gómez, C E; Georgian, Samuel E; Lunden, Jay J; Cordes, Erik E
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Contributor Yang, Yan
Publication Year 2017
Rights Creative Commons Attribution 3.0 Unported; https://creativecommons.org/licenses/by/3.0/
OpenAccess true
Representation
Language English
Resource Type Supplementary Dataset; Dataset
Format text/tab-separated-values
Size 16836 data points
Discipline Earth System Research
Spatial Coverage (-88.019W, 29.156S, -88.015E, 29.173N)
Temporal Coverage Begin 2014-08-19T00:00:00Z
Temporal Coverage End 2014-09-02T00:00:00Z