Phytoplankton species determination and counts determined from the community sampled at station West and East (PS97) before and after exposure to different Fe and Mn availabilities


Two Fe-Mn bottle amendment experiments with two natural phytoplankton communities were performed during Polarstern expedition PS97 in 2016 in the Drake Passage. At two locations, sea water was pumped (using trace metals clean techniques) from 25m depth and used to fill polycarbonate bottles after having passed through a cleaned 200 μm mesh (removing large grazers). The Control treatment was the sampled seawater without any trace metals addition while the other three treatments were enriched with either FeCl3 alone (0.5 nM; +Fe treatment) or MnCl2 alone (1 nM; +Mn treatment) or both trace metals together (+FeMn treatment). All treatments were done in triplicate 2,5L PC bottles. All incubation bottles were maintained at 30 μmol photons m-2 s-1 under a 16:8 (light:dark) hour cycle at 1 ̊C. To determine the effects of the different treatments on the microplankton composition for the two Fe-Mn enrichment experiments, unfiltered seawater was collected at the start and the end of both experiments for later analysis via light microscopy in the home laboratory. Briefly, samples were fixed with hexamine-buffered formalin solution (2% final concentration) and Lugol's solution (1% final concentration) and stored at 2 °C in the dark until taxonomic analysis. All samples were allowed to settle in Utermöhl sedimentation chambers for at least 24 hours and were analyzed on an inverted light microscope, according to the method of Utermöhl (1958). Species were counted and identified according to taxonomic literature. Each aliquot was examined until at least 400 cells had been counted.

Related Identifier
Metadata Access
Creator Balaguer, Jenna; Koch, Florian; Hassler, Christel S; Trimborn, Scarlett
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Publication Year 2021
Funding Reference German Science Foundation, 5472008
Rights Creative Commons Attribution 4.0 International;
OpenAccess true
Language English
Resource Type Dataset
Format text/tab-separated-values
Size 180 data points
Discipline Earth System Research
Spatial Coverage (-66.362W, -60.413S, -60.865E, -58.869N); Drake Passage; Scotia Sea
Temporal Coverage Begin 2016-03-02T08:19:00Z
Temporal Coverage End 2016-03-17T11:16:00Z