Seawater carbonate chemistry and behavioural abnormalities in coral reef fish, supplement to: Jarrold, Michael; Humphrey, Craig; McCormick, Mark I; Munday, Philip L (2017): Diel CO2 cycles reduce severity of behavioural abnormalities in coral reef fish under ocean acidification. Scientific Reports, 7(1)

DOI

Elevated CO2 levels associated with ocean acidification (OA) have been shown to alter behavioural responses in coral reef fishes. However, all studies to date have used stable pCO2 treatments, not considering the substantial diel pCO2 variation that occurs in shallow reef habitats. Here, we reared juvenile damselfish, Acanthochromis polyacanthus, and clownfish, Amphiprion percula, at stable and diel cycling pCO2 treatments in two experiments. As expected, absolute lateralization of A. polyacanthus and response to predator cue of Am. percula were negatively affected in fish reared at stable, elevated pCO2 in both experiments. However, diel pCO2 fluctuations reduced the negative effects of OA on behaviour. Importantly, in experiment two, behavioural abnormalities that were present in fish reared at stable 750 µatm CO2 were largely absent in fish reared at 750 ± 300 µatm CO2. Overall, we show that diel pCO2 cycles can substantially reduce the severity of behavioural abnormalities caused by elevated CO2. Thus, past studies may have over-estimated the impacts of OA on the behavioural performance of coral reef fishes. Furthermore, our results suggest that diel pCO2 cycles will delay the onset of behavioural abnormalities in natural populations.

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2018-09-04.

Identifier
DOI https://doi.org/10.1594/PANGAEA.893703
Related Identifier https://doi.org/10.1038/s41598-017-10378-y
Related Identifier https://doi.org/10.1111/mec.16124
Related Identifier https://doi.org/10.4225/28/5923bfed71f8d
Related Identifier https://cran.r-project.org/package=seacarb
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.893703
Provenance
Creator Jarrold, Michael; Humphrey, Craig; McCormick, Mark I; Munday, Philip L
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Contributor Yang, Yan
Publication Year 2017
Rights Creative Commons Attribution 3.0 Unported; https://creativecommons.org/licenses/by/3.0/
OpenAccess true
Representation
Language English
Resource Type Supplementary Dataset; Dataset
Format text/tab-separated-values
Size 26672 data points
Discipline Earth System Research
Spatial Coverage (146.667 LON, -18.400 LAT)
Temporal Coverage Begin 2015-07-01T00:00:00Z
Temporal Coverage End 2015-07-31T00:00:00Z